Tris(hydroxymethyl)aminomethane (CAS 77-86-1) – Wholesale & Research Grade
Tris is a primary amino compound that is tert-butylamine in which one hydrogen attached to each methyl group is replaced by a hydroxy group. A compound widely used as a biological buffer substance in the pH range 7--9; pKa = 8.3 at 20 ℃; pKa = 7.82 at 37 ℃. It has a role as a buffer. It is a triol and a primary amino compound. It is a conjugate base of a member of Htris.
Research Context
Tris(hydroxymethyl)aminomethane, commonly known as TRIS or THAM, has become an essential component in various biochemical and molecular biology applications. It serves primarily as a buffering agent, effectively maintaining pH stability during a range of experiments, including enzyme reactions, electrophoresis, and cell culture processes. Its ability to resist changes in pH under varying conditions makes it invaluable in the preparation of biological samples and in the formulation of reagents.
Mechanism of Action
The buffering capacity of TRIS arises from its ability to donate and accept protons, facilitating the maintenance of a constant pH environment. This characteristic is crucial in biological systems, where even slight deviations in pH can significantly impact enzyme activity and cellular processes. By providing a stable pH, TRIS ensures optimal conditions for biochemical reactions, thus enhancing the reliability of experimental outcomes.
Solubility and Storage Advice
Tris is highly soluble in water, making it easy to prepare various buffer solutions. It is advisable to store TRIS solutions at room temperature and protect them from light to prevent degradation. Additionally, for long-term storage, the powder form should be kept in a cool, dry place to maintain its integrity. Proper handling and storage are essential to ensure that TRIS maintains its buffering capacity and does not introduce variability in experimental results.
Future Research Directions
- Exploration of novel buffering systems that incorporate TRIS for enhanced stability in extreme conditions.
- Investigating the role of TRIS in developing sustainable bioprocessing techniques.
- Analyzing the effects of TRIS in various physiological and pathological contexts to better understand its potential therapeutic applications.
As research continues to evolve, TRIS remains a focal point for scientists seeking reliable methods to stabilize biochemical reactions, paving the way for advancements in both fundamental and applied research.
| CAS Number | 77-86-1 |
|---|---|
| Formula | C4H11NO3 |
| Mol. Weight | 121.14 g/mol |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| Grade | HPLC ≥98% |
Synthesis & Storage
Tris is supplied as a lyophilized powder to ensure stability during transit.
For long-term storage of CAS 77-86-1, we recommend maintaining at -20°C.
Researchers must reconstitute this peptide with bacteriostatic water or sterile solvent only when ready for use.
Quality Control: All batches undergo rigorous HPLC purity testing (≥98%) prior to dispatch from our USA fulfillment center.