Methylene Blue (CAS 61-73-4) – Wholesale & Research Grade
Methylene blue is an organic chloride salt having 3,7-bis(dimethylamino)phenothiazin-5-ium as the counterion. A commonly used dye that also exhibits antioxidant, antimalarial, antidepressant and cardioprotective properties. It has a role as an EC 1.4.3.4 (monoamine oxidase) inhibitor, an acid-base indicator, a fluorochrome, an antidepressant, a cardioprotective agent, an EC 3.1.1.8 (cholinesterase) inhibitor, a histological dye, an EC 4.6.1.2 (guanylate cyclase) inhibitor, an antioxidant, an antimicrobial agent, a neuroprotective agent, a physical tracer and an antimalarial. It contains a 3,7-bis(dimethylamino)phenothiazin-5-ium.
Research Context
Methylene Blue, also known as Methylthioninium chloride, is a synthetic dye with a wide array of research applications. Initially developed as a textile dye, it has gained prominence in various scientific fields, including microbiology, pharmacology, and neuroscience. Its utility ranges from staining biological tissues to serving as a redox indicator in chemical reactions. In recent years, Methylene Blue has been investigated for its potential therapeutic roles, particularly in treating conditions such as methemoglobinemia and Alzheimer's disease, where it may enhance cognitive function and neuroprotection.
Mechanism of Action
The mechanism of action of Methylene Blue is multifaceted. It is known to act as an electron donor in redox reactions, facilitating mitochondrial respiration and improving cellular energy metabolism. Additionally, it exhibits antioxidant properties, which help mitigate oxidative stress in neuronal cells. In the context of Alzheimer's disease, Methylene Blue has been shown to inhibit tau aggregation and reduce amyloid-beta toxicity, pointing to its potential as a neuroprotective agent.
Solubility and Storage
Methylene Blue is soluble in water and alcohol, making it versatile for laboratory applications. However, it is important to store it in a cool, dark place, away from light and moisture to prevent degradation. Properly sealed containers should be used to maintain its stability over time. When preparing solutions, it is advisable to use fresh stock and avoid prolonged exposure to air to preserve its potency.
Future Research Directions
Future research on Methylene Blue is poised to explore its therapeutic potential beyond its current applications. Studies are increasingly focusing on its role in mitochondrial dysfunction and neurodegenerative diseases. Additionally, the compound's effects on cellular signaling pathways and its application in novel drug formulations are areas ripe for exploration. As the scientific community continues to uncover its diverse capabilities, Methylene Blue may emerge as a key player in the development of innovative therapies for various health conditions.
| CAS Number | 61-73-4 |
|---|---|
| Formula | C16H18ClN3S |
| Mol. Weight | 319.9 g/mol |
| IUPAC Name | [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride |
| Grade | HPLC ≥98% |
Synthesis & Storage
Methylene Blue is supplied as a lyophilized powder to ensure stability during transit.
For long-term storage of CAS 61-73-4, we recommend maintaining at -20°C.
Researchers must reconstitute this peptide with bacteriostatic water or sterile solvent only when ready for use.
Quality Control: All batches undergo rigorous HPLC purity testing (≥98%) prior to dispatch from our USA fulfillment center.